Recently, the specific diagnostic reagent for bovine tuberculosis and the ELISA diagnostic kit undertaken by China Agricultural University have passed the identification organized by the Science and Technology Development Center of the Ministry of Education. It is understood that this project has conducted research on the development and application of bovine tuberculosis-specific diagnostic reagents and ELISA diagnostic kits. The research group has developed six specific proteins of Mycobacterium bovis strains, from which three specific diagnostic antigens of M. bovis Mycobacterium bovis Mce4E, RpfC and MPB83 with antigenicity have been screened. The indirect ELISA diagnostic method coated with recombinant fusion protein antigen and assembled into an indirect ELISA diagnostic kit. The bovine tuberculosis ELISA detection method is reliable, the performance of the detection reagent in the kit is stable, the specificity is 92%, and the sensitivity is 85%. The indirect ELISA diagnostic kit for bovine tuberculosis overcomes the shortcomings of traditional PPD such as time-consuming, laborious, and difficult to operate, and improves the specificity and sensitivity of the ELISA diagnostic kit. No domestic reports. The identification conclusion is: "1. The innovations of this achievement are as follows: 1) The Mce4 bovine Mce4 E and RpfC genes were cloned, expressed and purified, and these two proteins were used in the study of bovine tuberculosis ELISA diagnostic antigen. ) The use of three recombinant proteins of M. bovis Mce4E, RpfC and MPB83 to establish an indirect ELISA diagnostic method for bovine tuberculosis, assembling an indirect ELISA diagnostic kit with independent intellectual property rights that can be used to detect bovine tuberculosis at different stages of infection. The kit has strong specificity, high sensitivity, and stable performance. 3. The achievements have prominent social and economic benefits, which are of great significance in controlling bovine tuberculosis and ensuring food public health safety. For more information, please see the scientific and technological achievements of universities on this website Identification column.
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