When using the microscope, adopt the principle of focusing at a low magnification and high magnification. The focusing under the low magnification objective lens is equivalent to the initial focusing of the high magnification objective lens. When turning the high magnification objective lens, just turn the lens directly (ie, it does not change). The focal length is initially adjusted by low-power mirror operation. Under high magnification, the tissue can be observed with little or no adjustment. However, many operating instructions circumvent the specific meaning of "low magnification objective lens".
In the use of the microscope, the 10x objective lens is the standard objective lens used in the focusing work. The reason is that there is no sharp change from a 10x objective to a lower magnification objective, or a replacement from a 10x objective to a higher magnification objective. Another reason is that the focal length of the lower magnification objective lens is long, and the visual acuity of the observer is not easy to focus on, and the sample and the lens are easily touched when directly converted to the high magnification objective lens.
At the same time, the 10x objective lens is not only the common objective lens in the standard of focusing work, but also involves a lot of practical work. For example, in many national standards for metallographic examinations, the standard reference map for reference is the most common under 100-fold observation, and the 100-fold acquisition is a 10x objective with a 10x eyepiece. Starting from the actual operation, as long as it is not random or malicious, the previous operation should be such that the objective lens is in the vicinity of the focal plane. Under the condition of 10 times objective lens, after the sample is placed correctly, a blurred image should appear, even more clearly, slightly Adjust the fine adjustment.
(2) Relevant access
In the focusing problem after the conversion of the low magnification objective lens to the high magnification objective lens, our experience is quite different from the introduction in other literatures. Due to the improvement of the microscope manufacturing process, the collimation of different microscope microscopes is better, especially for foreign products. Thus, when the low-power focus is clear, it is clear that it is not necessary to refocus the image when going to high magnification observation; or The object distance can be increased slightly, and the degree of adjustment is by no means a concept of 1 to 3 turns, that is, the concept of 1 to 3 degrees (angle), and an extremely small adjustment amount.
(3) About the objective lens converter
When the objective lens is converted, the objective lens cannot be directly pushed by hand, otherwise the thread of the fixed objective lens may be loose and the optical axis may be skewed. The objective lens and microscopic digital camera system of the microscope are screwed onto the objective lens transducer. When changing the different objective lenses, rotate the objective lens converter to hear the slight “click†sound and the hand resistance increase to the end point. At this time, the objective lens is in the normal working position: perpendicular to the plane of the stage.
(4) The relationship between "shun, reverse" and "object distance"
The microscope coarsely adjusts and fine-tunes the direction of rotation of the knob, which is closely related to the increase and decrease of the object distance. The so-called clockwise, counterclockwise, is also relative, generally from the right side of the microscope to see the past effect; different models of the microscope, in the object distance reduction, increase the need to adjust the direction of the focus knob rotation, need to be in the teacher Clearly stated in the process of guidance. In the case of ambiguity, the relationship between the focus knob and the object distance must be known in advance when the microscope is officially operated; it must not be blindly based on the special occasions of clockwise or counterclockwise instructions.
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