3,4,5-trimethoxybenzoic acid:
P / N Inertex C18, 5u 150 × 4.6mm
Chromatographic conditions:
Mobile phase: 0.85mL 70% perchloric acid, 900mL water, 1g ammonium acetate, pH = 3, 1.54g heptane
Sodium sulfonate: acetonitrile = 20: 100: 880
Detection wavelength: 215nm Flow rate: 1.8mL / min
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