Rat Tumor Necrosis Factor α ELISA Kit Instructions
Detection range: 96T
10ng / L-360ng / L
purpose of usage:
The rat tumor necrosis factor alpha ELISA kit is used to determine the content of mouse tumor necrosis factor alpha (TNF-α) in rat serum, plasma and related liquid samples.
Experimental principle
This kit uses the double antibody sandwich method to determine the level of rat tumor necrosis factor alpha (TNF-α) in the specimen. Microporous plates were coated with purified rat tumor necrosis factor alpha (TNF-α) antibody to make solid-phase antibodies. Tumor necrosis factor alpha (TNF-α) was added to the microwells coated with monoclonal antibody in turn, followed by HRP The labeled tumor necrosis factor α (TNF-α) antibody binds to form an antibody-antigen-enzyme-labeled antibody complex, and after thorough washing, the substrate TMB is added for color development. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid. The color depth is positively correlated with the tumor necrosis factor alpha (TNF-α) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of rat tumor necrosis factor alpha (TNF-α) in the sample was calculated by a standard curve.
Kit composition
1
30 times concentrated washing solution
20ml × 1 bottle
7
Stop solution
6ml × 1 bottle
2
Enzyme reagent
6ml × 1 bottle
8
Standard product (720ng / L)
0.5ml × 1 bottle
3
Enzyme coated plate
12 holes × 8
9
Standard dilution
1.5ml × 1 bottle
4
Sample diluent
6ml × 1 bottle
10
Instructions
1 serving
5
Developer A liquid
6ml × 1 bottle
11
Sealing film
2 sheets
6
Developer B liquid
6ml × 1 / bottle
12
sealed bag
1
Specimen requirements
1. Specimens are extracted as soon as possible after collection, and extraction is performed according to relevant literature, and experiments should be conducted as soon as possible after extraction. If the test cannot be performed immediately, the specimen can be stored at -20 ℃, but repeated freezing and thawing should be avoided
2. The samples containing NaN3 cannot be detected because NaN3 inhibits horseradish peroxidase (HRP) activity.
Please refer to the attachment for the instruction manual of rat tumor necrosis factor α ELISA kit. You are welcome to call to order, business hotline, 13817140470.
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