Experimental principle of human mucosal addressin cell adhesion molecule (MAdCAM-1) ELISA Kit

Experimental principle of human mucosal addressin cell adhesion molecule (MAdCAM-1) ELISA Kit

1. Determination of mucosal addressin cell adhesion molecule (MAdCAM-1) ELISA Kit by double antibody sandwich method

Level.

2. First coat the microplate with purified human mucosal addressin cell adhesion molecule (MAdCAM-1) ELISA Kit antibody to make a solid phase antibody.

3. Then add human mucosal addressin cell adhesion molecule (MAdCAM-1) ELISA Kit to the microwells coated with mAb in turn, and then combine with HRP-labeled mucosal addressin cell adhesion molecule (MAdCAM-1) ELISA Kit antibody The antibody-antigen-enzyme-labeled antibody complex is thoroughly washed and added with substrate TMB for color development. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid.

4. The color depth is positively correlated with the mucosal addressin cell adhesion molecule (MAdCAM-1) ELISA Kit in the sample.

5. Finally, the absorbance (OD value) is measured with a microplate reader at a wavelength of 450 nm, and the concentration of the human mucosal addressin cell adhesion molecule (MAdCAM-1) ELISA Kit in the sample is calculated by the standard curve

.

Objective: To determine the content of human serum, plasma and related fluid samples of mucosal addressin cell adhesion molecule (MAdCAM-1) ELISA Kit.

5. Serum: The blood will naturally coagulate at room temperature for 10-20 minutes and centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully and centrifuge again if a precipitate appears during storage. Avoid any cell stimulation during the operation. Use test tubes free of pyrogens and endotoxins.

6. Plasma: EDTA or sodium citrate should be selected as the anticoagulant according to the requirements of the specimen. After mixing for 10-20 minutes, centrifuge for about 20 minutes (2000-3000 rpm) to remove particles. Collect the supernatant carefully, and centrifuge again if there is any precipitate.

safety:
1. Avoid direct contact with stop solution and substrate. If you accidentally come into contact with these liquids, please rinse them with water as soon as possible.
2. Do not eat, drink, smoke or use cosmetics during the experiment.
3. Do not touch any ingredients in the kit with your mouth.

4 Keep Elisa kit away from children

Bring your own items:

1. 37 ℃ incubator

2. Standard specification microplate reader

3. Precision pipette and disposable tip

4. Distilled water

5. Disposable test tube

6. Absorbent paper

Storage conditions and validity period:

1. Kit storage :; 2-8 ℃.

2. Validity: 6 months

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