Pollution control of CO2 incubator Figure 1 Neurons Figure 2 Red blood cells Figure 3 Human eggs
The CO2 incubator provides a stable culture environment for the in vitro culture of cells and tissues by controlling the temperature, humidity, and CO2 concentration. Widely used in mammalian cell culture, commonly used in cell dynamics research, collection of mammalian cell secretions, carcinogenic or toxicological effects of various physicochemical factors, research and production of antigens, culture of hybridoma cells to produce antibodies, in vitro fertilization, iPS inducible stem cells and other research fields.
In living organisms, organisms have their own immune system to protect cells or tissues, but when cultured in vitro, they do not have any immune barrier to protect themselves. For the basic parameters of incubator temperature, humidity and CO2 concentration, most incubators can meet the research needs. However, the control methods and effects of various incubators are different for various contaminants that cells face during the cultivation process, so the biggest threat in cell culture in vitro is actually the problem of pollution.
The main sources of pollution in CO2 incubators: bacteria, fungi (molds and yeasts), viruses, and mycoplasmas (Figure 4-7).
Figure 4 Bacteria Figure 5 Virus Figure 6 Mold Figure 7 Mycoplasma is also a very ideal culture environment for cells to survive these pollutants, the incubator itself will not be discerned, and 80% -90% of cell culture The time cells are located in the incubator, so whether the bacteriostasis or sterilization is very important!
Bacteria: After the bacteria are contaminated, the culture medium will change color in 1-2 days. The contaminated cells should be quickly isolated from other cell lines, discarded after sterilization, and the laboratory utensils and ultra-clean table should be disinfected with laboratory disinfectant to check HEPA filtration. Device.
Virus: Due to the parasitic survival of the virus, it is isolated from normal cells as soon as possible after the outbreak, and it is relatively easy to handle, but it poses a greater threat to the operator.
Fungi (moulds and yeasts): At present, there is no good suppression method, including amphotericin, which is commonly used now. Once the pollution is contaminated, it is easy to repeat repeatedly because spores are difficult to kill.
Mycoplasma: after being contaminated with mycoplasma, they can coexist with the cells for a long time because they will not cause cell death. The medium generally does not become turbid, the cells have no obvious changes, and the appearance gives a normal feeling. In fact, the cells have been potentially affected by many aspects, such as caused by Cell deformation, affecting DNA synthesis, inhibiting cell growth, etc.
Therefore, once these infections occur, the cells can only be discarded in the end, and cannot be recovered. The loss of many difficult experimental experiments is incalculable!
At present, the sterilization methods of CO2 incubators are mainly dry heat, damp heat, ultraviolet lamp irradiation, disinfectant wiping, etc.
High-temperature dry heat sterilization is recognized as an effective sterilization method; of course, wet heat sterilization can also achieve good sterilization effects under long-term conditions, but there are few wet heat sterilization boxes on the market now; ultraviolet lamp irradiation The effectiveness of the method is affected by many factors, such as occlusion, time, intensity, irradiation distance, humidity, etc., the sterilization effect is difficult to guarantee; the disinfectant wiping method is a method of sterilizing the surface, suitable for flat surfaces such as experimental countertops, The dead corners inside the cabinet, various devices due to the inability to wipe and other factors lead to a limited sterilization effect, and the disinfectants containing chlorine and iodine have a corrosive effect on stainless steel, and cannot be sterilized for the stainless steel cabinet.
The Thermo 8000 CO2 incubator in the United States is a representative of the high-quality incubators on the market today. It is sterilized by high temperature dry heat at 140 ° C and 120min to ensure that it can kill all microorganisms and fungal spores on the surface of the cabinet (Figure 8).
Figure 8 The Thermo 8000 CO2 incubator in the United States is sterilized at 140 ° C and 120min with dry heat and high temperature. In the design of the cabinet, the Thermo 8000 CO2 incubator in the United States has also been designed for bacteriostasis in many ways:
1) The inner wall is made of polished stainless steel (Figure 9), and research shows that polished stainless steel can significantly reduce the growth of pollutants;
Figure 9 Rounded design of polished stainless steel inner wall
2) HEAP high-efficiency air filter design (Figure 10), which continuously filters the air in the box, can effectively filter out the biological particles in the air, and guarantees that the air quality in the box reaches 100 grades (1 pc. Less than 100 air particles in cubic feet);
Figure 10 HEAP air filter
3) The silver ion water tray antibacterial box AquatecTM (Figure 11), the Thermo 8000 silver ion disinfection box AquatecTM only needs to be taken out of the packaging and put directly into the water tray to ensure that the water in the water tray does not grow bacteria and does not contain Chemical additives, to prevent pollution to the experiment, suitable for all kinds of water quality, even tap water, highly efficient and environmentally friendly, extremely low silver ion content, and even can be directly discharged, can be called a revolutionary disinfection product.
Figure 11 Silver ion disinfection box Therefore, the American Thermo 8000 CO2 incubator can not only provide an accurate in vitro simulation environment for cell growth, but also allow your cell culture to be safe and worry-free throughout the process. It is the best choice for cell culture!
American Thermo 8000 CO2 incubator (Figure 12-13)
Figure 12 Thermo 8000 direct heating CO2 incubator Figure 13 Control panel
Technical parameters and ordering information
CO2 control temperature accuracy ± 0.1 ℃
CO2 accuracy is better than ± 0.1%
range
+ 3 ℃ ~ + 50 ℃ (above room temperature), LED
range
0-20%
Uniformity ± 0.2 ℃ @ + 37 ℃
sensor
T / C or IR
Recovery Time
<10min, reach 37 ℃ (25 ℃, open the door for 30sec)
Recovery Time
<5min, reach 5 ± 0.2% (25 ℃, open the door for 30sec)
Dry heat sterilization, 140 ℃
Box structure alarm, power-on self-test, power-off restart, tracking alarm, over-temperature stop heating internal volume
184.1 liters of humidity inner wall stainless steel relative temperature ambient humidity to 95%
The inner door can be heated with a glass door. The water tray standard 3 liter water tray partition stainless steel, standard 4-layer display
1% incremental certification
UL, CE, ISO9001
Filter HEPA
Within 5 minutes after closing the door, the cavity reaches the level 100 clean index
3543 (infrared model)
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