Human gastric mucin (GM) ELISA kit for collecting samples, processing and storage methods:
1. Serum: Avoid any cell stimulation during the operation. Use test tubes free of pyrogens and endotoxins. After collecting blood, centrifuge at 1000 * g for 10 minutes to separate the serum and erythrocytes quickly and carefully
2. Plasma: EDTA, citrate, heparin plasma can be used for detection. Centrifuge at 1000 * g for 30 minutes to remove particles.
3. Cell supernatant: centrifuge at 1000 * g for 10 minutes to remove particles and polymer.
4. Tissue homogenate: Add tissue to the right amount of saline and mash. Centrifuge at 1000 * g for 10 minutes, and take the supernatant.
5. Storage: If the sample is not used immediately, it should be divided into small parts and stored at -70 ° C to avoid repeated freezing. As far as possible, do not use hemolysis to raise hyperlipidemia. If there are a large number of particles in the serum, centrifuge or filter before testing. Do not thaw at 37 ° C or higher. Thaw at room temperature and ensure that the sample is thawed evenly and adequately.
The company specializes in providing "human gastric mucin (GM) ELISA kit", a full range of products, quality assurance, free technical services:
Pre-sales: Provide customers with comprehensive technical consulting services and provide instructions, any consultation will be carefully answered, whether purchased or not;
After-sales: If there are quality problems with all elisa kits, they will be replaced free of charge. We will provide customers with sample test services of elisa kits (free of charge), and guarantee results within five working days. We will be patient for every technical problem. solve.
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