The company's professional enzyme-free services for many years: ELISA kits, standard products | reference materials, biological reagents, antibodies, culture media, serum, pharmaceutical intermediates, APIs and other scientific research reagents, quality assurance, low prices, national key laboratories and domestic The universities have long-term designated suppliers, with strong technical force and a group of professional technicians and sales personnel, providing comprehensive technical support and perfect after-sales service for our customers. Welcome to inquire!
Instruction manual for rat apolipoprotein E (Apo-E) elisa kit
Elisa kit specifications: 48-well configuration / 96-well configuration
Standard diluent: 1.5ml × 1 bottle
Enzyme label reagent: 3 ml × 1 bottle (48) / 6 ml × 1 bottle (96)
[Rat Apolipoprotein E (Apo-E) elisa kit] This reagent is for research use only
Calculation:
Taking the concentration of the standard as the abscissa and the OD value as the ordinate, draw a standard curve on the coordinate paper, and find the corresponding concentration from the standard curve according to the OD value of the sample; multiply by the dilution factor; or use the concentration of the standard Calculate the linear regression equation of the standard curve with the OD value, substitute the OD value of the sample into the equation, calculate the sample concentration, and multiply it by the dilution factor to obtain the actual concentration of the sample.
Kit composition:
Sealing film: 2 pieces (48) / 2 pieces (96)
Instructions: 1 copy
Sealed bag: 1
Standard product: 2700ng / L 0.5ml × 1 bottle 0.5ml × 1 bottle Store at 2-8 ℃
Enzyme-coated plate: 1 × 48 1 × 96 2-8 ℃
Sample diluent: 3ml × 1 bottle 6 ml × 1 bottle Store at 2-8 ℃
Developer A solution: 3ml × 1 bottle 6 ml × 1 bottle Store at 2-8 ℃
Developer B solution: 3ml × 1 bottle 6 ml × 1 bottle Store at 2-8 ℃
Stop solution: 3ml × 1 bottle, 6ml × 1 bottle, store at 2-8 ℃
Concentrated washing solution: (20ml × 20 times) × 1 bottle (20ml × 30 times) × 1 bottle stored at 2-8 ℃
Experimental principle:
This kit uses the double antibody sandwich method to determine the level of rat apolipoprotein E (Apo-E) in the specimen. Microporous plates were coated with purified rat apolipoprotein E (Apo-E) antibody to prepare solid-phase antibodies. Apolipoprotein E (Apo-E) was added to the microwells coated with monoclonal antibody in turn, followed by The labeled apolipoprotein E (Apo-E) antibody binds to form an antibody-antigen-enzyme-labeled antibody complex. After thorough washing, the substrate TMB is added for color development. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid. The color depth is positively correlated with apolipoprotein E (Apo-E) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of rat apolipoprotein E (Apo-E) in the sample was calculated by a standard curve.
Purpose: This kit is used to determine the content of apolipoprotein E (Apo-E) in rat serum, plasma and related liquid samples.
For such Chew toys, it is normally use TPR, of courses Rubber too, and it normally used for Dog training, like running, jumping, Biting etc. consider the reactive with the pets, some one used for slow feed ball, inside can out the food, snack etc pets loved, to give the pets reward after play, Also have the teeth cleaning function.
Dog Chew TPR Rubber Toys,TPR Pet Chew Toys,Rubber Bone Pet Toy,Dog Chew Rope Toys
NINGBO DAC ARTS & CRAFTS COMPANY , http://www.dacpets.com